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Darline attended Anderson College (now University) for two years and then went on to earn her bachelor’s degree from the College of Charleston. She later earned a Master’s degree in rehabilitation counseling from South Carolina State University in Orangeburg, a Historically Black College and University (HBCU). Her colleague, Senator Tim Scott, pointed out that she is the only current senator serving with a degree from an HBCU. Her first job was working in an optometrist’s office, eventually earning her optician’s certification. She also worked at Clemson University and as a rural carrier associate with the United States Postal Service.